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KMID : 0380220060390040383
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Journal of Biochemistry and Molecular Biology
2006 Volume.39 No. 4 p.383 ~ p.393
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Characteristics of Thiamine Uptake by the BeWo Human rophoblast Cell Line
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Keating Elisa
Lemos Clara
Azevedo Isabel
Martel Fatima
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Abstract
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Little is known concerning the mechanisms responsible for the transplacental transfer of thiamine. So, the aim of this work was to characterize the placental uptake of thiamine from the maternal circulation, by determining the characteristics of 3H-thiamine uptake by a human trophoblast cell line (BeWo). Uptake of 3H-thiamine (50-100 nM) by BeWo cells was: 1) temperature-dependent and energy-independent; 2) pH-dependent (uptake increased as the extracellular medium pH decreased); 3) Na+- dependent and Cl--independent; 4) not inhibited by the thiamine structural analogs amprolium, oxythiamine and thiamine pyrophosphate; 5) inhibited by the unrelated organic cations guanidine, N-methylnicotinamide, tetraethylammonium, clonidine and cimetidine; 6) inhibited by the organic cation serotonin, and by two selective inhibitors of the serotonin plasmalemmal transporter (hSERT), fluoxetine and desipramine. We conclude that 3H-thiamine uptake by BeWo cells seems to occur through a process distinct from thiamine transporter-1 (hThTr-1) and thiamine transporter-2 (hThTr-2). Rather, it seems to involve hSERT. Moreover, chronic (48 h) exposure of cells to caffeine (1 μM) stimulated and chronic exposure to xanthohumol and iso-xanthohumol (1 and 0.1 μM, respectively) inhibited 3H-thiamine uptake, these effects being not mediated through modulation of the expression levels of either hThTr-1 or hSERT mRNA.
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KEYWORD
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Apical uptake, BeWo cells, Ethanol, Methylxanthines, Polyphenols, Thiamine
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